The Journal of Experimental Medicine
Symposium on Dendritic Cells
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The Journal of Experimental Medicine, Vol 137, 1263-1274, Copyright © 1973 by The Rockefeller University Press

ARTICLE

SYNTHESIS OF ENDOGENOUS PYROGEN BY RABBIT LEUKOCYTES

Douglas M. Moore 1, Patrick A. Murphy 1, P. Joan Chesney 1, and W. B. Wood Jr. 1

1 From the Department of Microbiology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205

Rabbit ieukocytes from peritoneal exudates and from blood were stimulated to form leukocyte pyrogen in the presence of radiolabeled amino acids. The stimuli used were endotoxin, phagocytosis, and tuberculin. The crude leukocyte pyrogen samples were purified; pyrogen from exudate cells was rendered homogeneous; pyrogen from blood cells was still contaminated with other proteins. All the purified pyrogens were radioactive; and for all it was shown that radioactivity and pyrogenic activity coincided on electrophoresis at pH 3.5 and pH 9 in acrylamide and on isoelectric focusing in acrylamide. Furthermore, pyrogens obtained from exudate cells stimulated in different ways, or from blood cells and exudate cells stimulated with endotoxin, appeared to be identical. These results suggest that leukocyte pyrogen was synthesized de novo from amino acid precursors and that leukocytes made the same pyrogen whatever the stimulus used to activate them.

Submitted on January 15, 1973


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