The Journal of Experimental Medicine
Cytokines Montreal 2008
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The Journal of Experimental Medicine, Vol 137, 1124-1141, Copyright © 1973 by The Rockefeller University Press

ARTICLE

IMMUNOINHIBITION OF INTRACELLULAR PROTEIN DIGESTION IN MACROPHAGES

John T. Dingle 1, A. Robin Poole 1, Gerald S. Lazarus 1, and Alan J. Barrett 1

1 From the Tissue Physiology Department, Strangeways Research Laboratory, Cambridge CB1 4RN, England

Specific anti-(rabbit cathepsin D) serum, previously shown to inhibit cathepsin D, arrested the intracellular digestion of sheep IgG and radiochemically labeled hemoglobin and proteoglycan in rabbit alveolar macrophages. In the presence of antiserum, cells remained viable, but became very vacuolated. Both sheep IgG and hemoglobin were demonstrated immunocytochemically in vacuoles most of which could also be shown to contain cathepsin D. When the antiserum was removed, cells regained their normal morphology, and digestion of endocytosed proteins returned to normal. These results indicate that cathepsin D can be inhibited within lysosomes of viable cells, in which it plays a major role in the intracellular digestion of certain proteins.

Submitted on December 17, 1972


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