The Journal of Experimental Medicine, Vol 137, 511-526,
Copyright © 1973 by The Rockefeller University Press
INDEPENDENCE OF H-2K AND H-2D ANTIGENIC DETERMINANTS ON THE SURFACE OF MOUSE LYMPHOCYTES
Catherine Neauport-Sautes 1,
Frank Lilly 1,
Danielle Silvestre 1, and
François M. Kourilsky 1
1 From the Laboratoire d'Immunologie des Tumeurs, Institut de Recherches sur les Maladies du Sang, Hôpital Saint Louis, 75010 Paris, France, and the Department of Genetics, Albert Einstein College of Medicine, Bronx, New York 10461
At 37°C, fluorescein-conjugated anti-H-2 alloantibodies specifically induce, at the surface of living mouse lymphocytes, the redistribution of the corresponding H-2 antigens, which cluster as patches and sometimes single caps at one pole of the cell. This aggregation is inhibited at 0°C and the H-2 antigens, stained by fluorescent antibodies in the cold, appear evenly spread over the cell surface.
This phenomenon was used to define the relationships between the membrane structures bearing the antigens coded by the H-2K and the H-2D genes of the H-2 region. Monospecific anti-H-2 antibodies coupled to either tetramethyl rhodamine isothiocyanate or fluorescein isothiocyanate were used to induce the redistribution of H-2D and H-2K antigens of the H-2b and H-2k haplotype at the surface of lymph node cells from homozygous and F1 hybrid mice. It was observed that the diffuse distribution of H-2K antigens labeled at 0°C was not affected by the prior antibody-induced aggregation of H-2D antigens and vice versa. The results were the same for H-2 antigens governed by genes located either in cis or in trans position.
These data indicate that the H-2K and H-2D antigens migrate independently at the cell surface, and suggest that the gene products from the D and the K end of the H-2 region are expressed on independent molecules or structures at the cell membrane.
Submitted on October 11, 1972
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