¹ From the National Institute for Medical Research, Mill Hill, London NW7 IAA, and the Medical Research Council Neuroimmunology Project, Department of Zoology, University College London, London WC1E 6BT, England

Anti-AKR antibody conjugated to fluorescein has been used in direct immunofluorescence tests to identify spleen ⁺ (T) sheep erythrocyte rosette-forming cells in AKR mice. Specificity studies involving A and cogenic A/AKR mice clearly demonstrated that the cell surface fluorescence and cytotoxicity produced by the antiserum is directed solely toward the AKR alloantigen. Approximately of rosette-forming and non-rosette-forming spleen cells were found to be ⁺. The tendency for T cells to bind less antigen and the tendency for antigen-binding T cells to bear less than other spleen T cells, first suggested by other studies involving rosette-elimination by anti-C3H plus complement, were confirmed by direct immunofluorescence. All AKR rosettes are specifically inhibitable by anti-immunoglobulin, including T rosettes. Antigen-induced redistribution of T cell receptors, analogous to that previously described for B cell receptors (16), occurs as readily in ⁺RFC as in ^- RFC, without altering the symmetrical ring distribution of AKR antigen.

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This article has been cited by other articles:

• Marchalonis, J. (1975). Lymphocyte surface immunoglobulins. Science 190: 20-29 [Abstract]  
• Sell, S., Sheppard, H. W. Jr. (1973). Rabbit Blood Lymphocytes May Be T Cells with Surface Immunoglobulins. Science 182: 586-587 [Abstract]  

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