The Journal of Experimental Medicine, Vol 137, 22-31,
Copyright © 1973 by The Rockefeller University Press
EVIDENCE FOR THE LINKAGE OF THE IGCH LOCUS TO A GENE CONTROLLING THE IDIOTYPIC SPECIFICITY OF ANTI-p-AZOPHENYLARSONATE ANTIBODIES IN STRAIN A MICE
Laura L. Pawlak 1,
Elizabeth B. Mushinski 1,
Alfred Nisonoff 1, and
Michael Potter 1
1 From the Department of Biological Chemistry, University of Illinois, College of Medicine, Chicago, Illinois 60680, and the Laboratory of Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014
Anti-p-azophenylarsonate (anti-Ar) antibodies elicited in all strain A/J mice tested share one or more idiotypic specificities. These specificities are also found in the anti-Ar antibodies of mice of the closely related strain, AL/N, but not in those of BALB/c mice.
Anti-Ar antibodies were elicited in congenic mice in which the IgCH locus of AL/N mice, which controls allotypic markers in the constant regions of heavy chains, had been introgressively backcrossed for nine generations onto a BALB/c background; the mice were then rendered homozygous for the AL/N allotypic determinant. On the average, these antibodies were quantitatively equivalent, with respect to content of the cross-reactive idiotype, to those of AL/N mice. This indicates that the gene controlling the idiotype is closely linked to the IgCH locus. Since idiotype must be a function of V region sequences, the results suggest close linkage of VH and CH genes. The cross-reactive idiotype was found in nearly all F1 mice (C57/BL x A/J or BALB/c x A/J) tested.
Submitted on September 11, 1972
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