The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 136, 1043-1053, Copyright © 1972 by The Rockefeller University Press

ARTICLE

STUDIES IN PORPHYRIA : I. A DEFECT IN THE REDUCTIVE TRANSFORMATION OF NATURAL STEROID HORMONES IN THE HEREDITARY LIVER DISEASE, ACUTE INTERMITTENT PORPHYRIA



Attallah Kappas 1, H. Leon Bradlow 1, Peter N. Gillette 1, and T. F. Gallagher 1

1 From The Rockefeller University, New York 10021, and The Institute for Sleroid Research, Montefiore Hospital, Bronx, New York 10467

A variety of 5ß steroid metabolites derived from hormones natural to man are potent inducers experimentally of delta-aminolevulinate synthetase, the rate-limiting enzyme in porphyrin-heme formation. This mitochondrial enzyme is found at high levels of activity in the livers of patients with the genetic disease, acute intermittent porphyria (AIP). In this study the metabolism of 14C-labeled testosterone was examined in AIP patients to determine whether there was a disproportionate conversion of the hormone to its 5ß, compared to its 5alpha metabolite. The results indicate that AIP subjects do generate a substantially greater than normal fraction of 5ß metabolite from this steroid; the excessive degree of ring A reduction of testosterone taking place via the 5ß pathway in the porphyric patients averages 350% greater than in the nonporphyric subjects. In one asymptomatic AIP patient the disproportionate generation of 5ß metabolite from the hormone reached a level 10 times the normal mean. Studies with a second 14C-labeled hormone, dehydroisoandrosterone, whose metabolism in man resembles that of testosterone, confirmed the derangement in reductive transformation of steroids found in the individuals carrying the genetic lesion of AIP.

These findings define a new endocrine abnormality in AIP patients and raise the possibility that endogenously derived 5ß steroids may contribute by an induction mechanism to the increased levels of hepatic delta-aminolevulinate synthetase activity found in AIP patients.

 Submitted on June 7, 1972


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