M. Jondal ¹, G. Holm ¹, and H. Wigzell ¹

¹ From the Department of Tumour Biology, Karolinska Institutet, and the Seraphimer Hospital, Stockholm, Sweden

By using the two criteria (a) high density of immunoglobulin determinants on the cell surface and (b) presence of receptors for C'3 on the cell surface for defining bone marrow-derived lymphocytes, it is indirectly shown that all or at least a major population of human thymus-derived lymphocytes under certain conditions will form nonimmune rosettes with sheep red blood cells (SRBC). Almost all thymocytes tested from two different donors formed rosettes. The SRBC rosettes are not formed by virtue of immunoglobulin receptors and form only around living cells. Positive bivalent ions are required for rosette formation since EDTA will block rosette formation. Sodium iodoacetate will also block rosette formation demonstrating the dependence on an intact glycolytic pathway. Rosette formation is temperature dependent and will not appear at 37°C. Trypsin treatment of lymphocytes will abolish their SRBC-binding ability which cannot be restored by treating them with fresh donor serum or fetal calf serum, but which will reappear after culturing the lymphocytes. It is suggested that these rosettes are formed by a rapidly released or metabolized receptor substance on the living cell surface which behaves as a trypsin-sensitive structure produced by the cells themselves.

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

• Monday, S. R., Vath, G. M., Ferens, W. A., Deobald, C., Rago, J. V., Gahr, P. J., Monie, D. D., Iandolo, J. J., Chapes, S. K., Davis, W. C., Ohlendorf, D. H., Schlievert, P. M., Bohach, G. A. (1999). Unique Superantigen Activity of Staphylococcal Exfoliative Toxins. J. Immunol. 162: 4550-4559 [Abstract] [Full Text]  
• Williamson, M. T., Murti, P. K. (1996). Effects of Storage, Time, Temperature, and Composition of Containers on Biologic Components of Human Milk. J Hum Lact 12: 31-35 [Abstract]  
• Reinherz, E.L., Chang, H.-C., Clayton, L.K., Gardner, P., Howard, F.D., Koyasu, S., Jin, Y.-J., Moingeon, P., Sayre, P.H. (1989). The Biology of Human CD2. Cold Spring Harb Symp Quant Biol 54: 611-625 [Abstract]  
• Moriguchi, S., Jacksont, J.C., Watson, R.R. (1985). Effects of Retinoids on Human Lymphocyte Functions in vitro. Hum Exp Toxicol 4: 365-378 [Abstract]  
• Ninnemann, J. L., Stratta, R. J., Warden, G. D., Saffle, J. R., Weber, M. E. (1984). The Effect of Plasma Exchange on Lymphocyte Suppression After Burn. Arch Surg 119: 33-38 [Abstract]  
• Mullin, T. J., Kirkpatrick, J. R. (1983). Substrate Composition and Sepsis: Effects on Immunity. Arch Surg 118: 176-180 [Abstract]  
• Stern, M.H., Dreizen, S., Mackler, B.F., Levy, B.M. (1982). Isolation and Characterization of Inflammatory Cells from the Human Periapical Granuloma. JDR 61: 1408-1412 [Abstract]  
• Edstrom, S., Jacobsson, S., Jeppsson, P.-H. (1979). Mucosal Melanoma: Immunological Findings in a Rare Case Treated With BCG Vaccine, Autologous Tumor Cells, and Cytarabine. Arch Otolaryngol Head Neck Surg 105: 48-50 [Abstract]  
• Bach, M. L., Huang, S.-W., Hong, R., Poulik, M. D. (1973). beta2-Microglobulin: Association with Lymphocyte Receptors. Science 182: 1350-1352 [Abstract]  
• Kersey, J. H., Sabad, A., Gajl-Peczalska, K., Hallgren, H. M., Yunis, E. J., Nesbit, M. E. (1973). Acute Lymphoblastic Leukemic Cells with T (Thymus-Derived) Lymphocyte Markers. Science 182: 1355-1356 [Abstract]  
• Sell, S., Sheppard, H. W. Jr. (1973). Rabbit Blood Lymphocytes May Be T Cells with Surface Immunoglobulins. Science 182: 586-587 [Abstract]  

Home | Help | Feedback | Subscriptions | Archive | Search

TABLE OF CONTENTS