POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS: I. THE RESPONDING CELL

doi:10.1084/jem.136.1.128

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ARTICLE

POTENTIATION OF THE T-LYMPHOCYTE RESPONSE TO MITOGENS : I. THE RESPONDING CELL

Igal Gery ¹, Richard K. Gershon ¹, and Byron H. Waksman ¹

¹ From the Departments of Microbiology and Pathology, Yale University School of Medicine, New Haven, Connecticut 06510

Human and mouse lymphoid cells, stimulated by phytohemagglutinin(PHA) or lipopolysaccharide W (LPS), release supernatant factor(s)which are mitogenic for mouse thymocytes and which potentiatetheir responses to PHA or concanavalin A (Con A), The term LAF(lymphocyte-activating factor) is proposed for this activity.LAF not only enhances the mitotic responses of the less densethymus subpopulations (A, B, and C) separable on discontinuousbovine serum albumin (BSA) gradients but also gives substantialresponses in the otherwise inert cells of the denser fractionsD and P. LAF does not exert a potentiating stimulatory effecton the responses of unfractionated mouse spleen cells, but doesact synergistically with PHA on nonadherent spleen cells andon spleen cells of mice of several strains 5 days after irradiationand injection of thymocytes. Similarly LAF, which has no visibleeffect on unfractionated human peripheral blood cells, stronglypotentiates the PHA response of column-purified lymphocytes,when these are cultured at low concentration. We conclude thatLAF stimulates both central and peripheral T lymphocytes andenhances their responses to other stimulants.

Submitted on February 24, 1972

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