The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 135, 1410-1415, Copyright © 1972 by The Rockefeller University Press

BRIEF DEFINITIVE REPORTS

CLASSIFICATION OF THYMUS-DERIVED AND MARROW-DERIVED LYMPHOCYTES BY DEMONSTRATION OF THEIR ANTIGEN-BINDING CHARACTERISTICS

J. S. Haskill 1, B. E. Elliott 1, R. Kerbel 1, M. A. Axelrad 1, and D. Eidinger 1

1 From the Department of Pathology, Queen's University and Kingston General Hospital, and the Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada

Antigen-binding cells of T and B origin can readily be determined by quantitating the number of sheep erythrocytes per rosette after glutaraldehyde fixation. The T1 and T2 populations have low antigen-binding properties and are very unstable without fixation. The B1 and B2 populations are stable and correlate with precursor and secretory cells. Fixation of rosettes permits a sensitive test for studying differentiation of T and B cells.

Submitted on April 2, 1972


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