The Journal of Experimental Medicine
Janeway's Immunobiology 7th Edition
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The Journal of Experimental Medicine, Vol 135, 1247-1258, Copyright © 1972 by The Rockefeller University Press

ARTICLE

IMMUNOCHEMICAL STUDIES ON BLOOD GROUPS : LIV. CLASSIFICATION OF ANTI-I AND ANTI-i SERA INTO GROUPS BASED ON REACTIVITY PATTERNS WITH VARIOUS ANTIGENS RELATED TO THE BLOOD GROUP A, B, H, LEa LEb AND PRECURSOR SUBSTANCES



Ten Feizi 1 and Elvin A. Kabat 1

1 From the Departments of Microbiology, Neurology, and Human Genetics and Development, College of Physicians and Surgeons, Columbia University; the Neurological Institute, Presbyterian Hospital, New York 10032; and the Rockefeller University, New York 10021

Quantitative precipitin assays were carried out with eleven anti-I and five anti-i cold agglutinin sera. Their reaction patterns with precursor substances and with certain antigens related to the A, B, H, Lea, and Leb substances revealed at least six types of I and four types of i specificity. All of the I and i determinants appear to be represented in varying amounts on that fraction of the ovarian cyst precursor substance OG which was precipitated by 20% ethanol (OG 20% 2X). Only some of these determinants can be detected in the first periodate oxidation stages of A, B, and H substances, in cow substances, and in hydatid cyst fluid.

Submitted on February 17, 1972


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