A PREALBUMIN ACTIVATOR OF PREKALLIKREIN: III. APPEARANCE OF CHEMOTACTIC ACTIVITY FOR HUMAN NEUTROPHILS BY THE CONVERSION OF HUMAN PREKALLIKREIN TO KALLIKREIN

doi:10.1084/jem.135.1.81

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A PREALBUMIN ACTIVATOR OF PREKALLIKREIN : III. APPEARANCE OF CHEMOTACTIC ACTIVITY FOR HUMAN NEUTROPHILS BY THE CONVERSION OF HUMAN PREKALLIKREIN TO KALLIKREIN

Allen P. Kaplan ¹, A. B. Kay ¹, and K. Frank Austen ¹

¹ From the Departments of Medicine, Harvard Medical School and the Robert B. Brigham Hospital, Boston, Massachusetts 02120

Human plasma kallikrein has been shown to directly and selectivelyattract human neutrophils from a mixed leukocyte population.The capacity of plasma kallikrein to be chemotactic and to generatethe nonapeptide bradykinin was maintained during progressivepurification. While neither highly purified prekallikrein northe prealbumin Hageman factor fragments were chemotactic alone,their interaction so as to convert prekallikrein to kallikreinyielded both chemotactic and kinin-generating activity. Bothfunctions of kallikrein were inhibited by treatment with diisopropylfluorophosphate, indicating an essential role for the activesite of the enzyme in the expression of its chemotactic activity.

Submitted on September 7, 1971

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