The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gudat, F. G.
Right arrow Articles by Hummeler, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gudat, F. G.
Right arrow Articles by Hummeler, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 134, 1155-1169, Copyright © 1971 by The Rockefeller University Press

ARTICLE

STUDIES ON ANTIBODY-PRODUCING CELLS : III. IDENTIFICATION OF YOUNG PLAQUE-FORMING CELLS BY THYMIDINE-3H LABELING



Fred G. Gudat M.D.1, T. N. Harris M.D.1, Susanna Harris Ph.D.1, and Klaus Hummeler M.D.1

1 From The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania 19146, and the Department of Pediatrics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104

Mice injected with sheep RBC and then, 4 days later with thymidine-3H, were sacrificed on the day of thymidine-3H injection or 1 or 2 days later. Hemolytic antibody plaque preparations were made of cells from the draining lymph nodes by a thin-plating procedure permitting collection of isolated PFC for electron microscopic examination and radioautography.

Of cells obtained on the day of thymidine-3H injections, 65% of the labeled PFC were in the lymphocytic category, in comparison with 13% found previously in the entire population of such cells. The remaining 35% were plasmablasts in early stages of differentiation. Cells obtained 1 day after the thymidine-3H injections showed a shift to a majority of labeled cells in the plasmacytic category. Also, the plasmablasts were substantially more differentiated than those of the previous day, and some mature plasma cells were now seen. The labeled PFC obtained on day 2 gave no indication of further differentiation.

Cells of rabbit lymph nodes labeled in vitro with thymidine-3H showed a range of labeled PFC. The majority were in the plasmacytic category, including some mature plasma cells.

The data from the experiments with in vivo labeling suggest a direct differentiation from antibody-synthesizing lymphocytes to plasma cells. Further, the in vivo experiments indicated that differentiation from nascent lymphocyte to plasma cell could be essentially completed within 1 day.

 Submitted on June 21, 1971


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS