The Journal of Experimental Medicine
Keystone Symposia
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The Journal of Experimental Medicine, Vol 134, 495-516, Copyright © 1971 by The Rockefeller University Press

ARTICLE

RECEPTORS ON IMMUNOCOMPETENT CELLS : II. SPECIFICITY AND NATURE OF RECEPTORS ON DINITROPHENYLATED GUINEA PIG ALBUMIN-125I-BINDING LYMPHOCYTES OF NORMAL GUINEA PIGS



Joseph M. Davie M.D.1 and William E. Paul M.D.1

1 From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Nonimmunized guinea pigs possess rare lymphocytes which bind sufficient 2,4-dinitrophenyl-guinea pig albumin-125I (DNP-GPA) to their surface to be detected by short-term radioautography. The cells occur in the lymph nodes, spleen, peripheral blood, and bone marrow with a frequency of sim40/100,000 lymphocytes, but are absent from the thymus. The receptors of these cells are largely specific for the haptenic group (epsi-DNP-L-lysine) as shown by inhibition of DNP-GPA-125I binding with epsi-DNP-L-lysine and with DNP bovine serum albumin (DNP-BSA). Furthermore, these cells specifically adsorb to agarose beads to which either DNP-GPA, DNP-BSA, or DNP-keyhole limpet hemocyanin (KLH) has been covalently linked. This hapten specific depletion of DNP-GPA-125I antigen-binding cells (ABC) correlates with a similar diminution in the capacity of adsorbed populations to transfer primary responsiveness to DNP-KLH to irradiated syngeneic recipients.

Fluoresceinated anti-immunoglobulin binds to the surface of some guinea pig lymphocytes, and all DNP-GPA-125I ABC, as shown by a double-label technique. The great majority of DNP-GPA ABC and human gamma-globulin ABC possess surface Ig molecules of the gamma2 heavy chain class. Preincubation of cell suspensions with anti-gamma2 antibody markedly diminishes the number of DNP-GPA-125I ABC which are detected, strongly suggesting that the receptors of these cells are immunoglobulin molecules, most of which possess gamma2 heavy chains.

The specificity characteristics of DNP-GPA-125I ABC are strikingly different from those of cells mediating a cellular immune response to DNP-GPA, indicating major differences in the specificity and nature of the receptors of these cell types.

 Submitted on April 6, 1971


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