INFECTION AND TRANSFORMATION OF MOUSE PERITONEAL MACROPHAGES BY SIMIAN VIRUS 40

doi:10.1084/jem.134.2.335

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INFECTION AND TRANSFORMATION OF MOUSE PERITONEAL MACROPHAGES BY SIMIAN VIRUS 40

J. Mauel Ph.D.¹ and V. Defendi M.D.¹

¹ From The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

The stimulation of DNA synthesis in mouse (C57BL) macrophagesexplanted in vitro was demonstrated after treatment with conditionedmedium or infection with SV40. In the latter case, inductionof SV40 T antigen was detected before TdR-³H incorporation.Even though all macrophages were infected (T antigen-positive),they exhibited considerable pleomorphism, accompanied by functionaldifferences. Permanent lines of SV40-transformed macrophageswere eventually established, and one clone was isolated whichreplicates indefinitely and has many properties of primary macrophages:high acid phosphatase and phagocytic activity, lysozyme production,and specific antigenic determinants. These cells differ fromnormal macrophages in that they contain the SV40 genome, canbe trypsinized, and do not require conditioned medium for continuedreplication.

Submitted on March 15, 1971

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