The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sherr, C. J.
Right arrow Articles by Uhr, J. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sherr, C. J.
Right arrow Articles by Uhr, J. W.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH
Hazardous Substances DB
*TRITIUM
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 133, 901-920, Copyright © 1971 by The Rockefeller University Press

ARTICLE

IMMUNOGLOBULIN SYNTHESIS AND SECRETION : VI. SYNTHESIS AND INTRACELLULAR TRANSPORT OF IMMUNOGLOBULIN IN NONSECRETORY LYMPHOMA CELLS



Charles J. Sherr 1 and Jonathan W. Uhr M.D.1

1 From the Irvington House Institute and the Department of Medicine, New York University School of Medicine, New York 10016

Cells from an established line of Burkitt's lymphoma (Daudi) and a mouse myeloma (P3K) were pulse-labeled in vitro with 3H-leucine, and immunoglobulin was immunologically precipitated from cell lysates and secretions. In contrast to P3K cells, Daudi cells synthesize a small amount of Ig which is not secreted. Subcellular fractionation experiments indicated that Ig of Daudi cells is synthesized on membrane-bound polyribosomes and enters the cisternae of the microsomes. Ig in the microsomes could be labeled with either 3H-galactose or 3H-fucose suggesting that transport proceeds to the Golgi complex. Additional evidence indicates that Ig molecules are transported to the plasma membrane but are not cleaved from the cell surface. These results together with other studies of Burkitt lymphoma cells suggest that the Daudi line may represent a clone of neoplastic cells derived from normal lymphocytes which synthesize but do not secrete Ig. Similarities between lymphoma cells and antigen-binding cells are discussed.

Submitted on November 30, 1970


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS