THE PHLOGISTIC ROLE OF C3 LEUKOTACTIC FRAGMENTS IN MYOCARDIAL INFARCTS OF RATS

doi:10.1084/jem.133.4.885

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ARTICLE

THE PHLOGISTIC ROLE OF C3 LEUKOTACTIC FRAGMENTS IN MYOCARDIAL INFARCTS OF RATS

Jeffrey H. Hill ¹ and Peter A. Ward M.D.¹

¹ From the Immunobiology Branch, Armed Forces Institute of Pathology, Washington, D. C. 20305

An improved method is described for extraction from rat myocardiumof an enzyme that cleaves the third component of complement(C3) into leukotactic fragments. Direct cleavage of C3 has beenshown by the use of a radiolabeled preparation of human C3.The enzyme elutes slightly beyond (retarded to) an albumin markerin gel filtration.

After surgical ligation of a coronary artery,leukotactic activity can be extracted from infarcted rat myocardium.Described in terms of its time of appearance in infarcted tissue,the leukotactic activity has been identified as cleavage productsof rat C3. The C3 fragments are of low molecular weight andheterogenous in size.

Rats, if treated with the C3 inactivator(isolated from cobra venom) so as to ablate serum C3, fail todevelop leukotactic activity in the infarcted myocardium. Inturn, few if any of the usual intense accumulations of neutrophilsin response to infected myocardium are seen during the first48 hr after coronary artery ligation.

These studies suggesta nonimmunologic role for C3 in the mediation of the acute inflammatoryresponse in nonspecific tissue injury.

Submitted on October 8, 1970

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