The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 133, 752-771, Copyright © 1971 by The Rockefeller University Press

ARTICLE

IGE AND IGGA ANTIBODY-MEDIATED RELEASE OF HISTAMINE FROM RAT PERITONEAL CELLS : I. OPTIMUM CONDITIONS FOR IN VITRO PREPARATION OF TARGET CELLS WITH ANTIBODY AND CHALLENGE WITH ANTIGEN



Michael K. Bach Ph.D.1, Kurt J. Bloch M.D.1, and K. Frank Austen M.D1

1 From the Department of Medicine, Robert B. Brigham Hospital, the Clinical Immunology and Arthritis Units, Department of Medicine, Massachusetts General Hospital, and the Department of Medicine, Harvard Medical School, Boston, Massachusetts 02120

The optimum conditions for antigen-induced release of histamine in the rat IgE and IgGa antibody-mediated systems were studied in vitro. The IgE antibody-mediated reaction could be separated into two steps: preparation of target cells with antibody and challenge with antigen. The optimal conditions for these two steps were distinctly different. Release of histamine by IgGa antibody and antigen could not be separated into two steps, and the optimal conditions for the total reaction were identical to those of the antigen challenge step of the IgE antibody-mediated system.

Submitted on November 4, 1970


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