Enrique Rabellino M.D.¹, Sonia Colon ¹, Howard M. Grey M.D.¹, and Emil R. Unanue M.D.¹

¹ From the Department of Allergy and Clinical Immunology, National Jewish Hospital and Research Center, Denver, Colorado 80206 and the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

The distribution, and quantity of immunoglobulins on the surface of lymphocytes has been studied by means of immunofluorescence and a quantitative radio-immunoassay. Surface immunoglobulins were found on approximately 45% of spleen and marrow lymphocytes and 7–14% of lymphocytes from lymph nodes, peripheral blood, and peritoneal exudate. Thymic lymphocytes contained undetectable amounts of immunoglobulin. In the spleen the different immunoglobulins were present in the following order: G2 > G1 > M > A > G3. The surface immunoglobulin was largely removable by brief treatment with trypsin. Quantitative analysis indicated that 50,000–150,000 molecules of immunoglobulin were present on an individual cell. A variety of observations make it likely that this lymphocyte-associated immunoglobulin. is a product of the cell to which it is attached rather than a form of cytophilic antibody.

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