CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES: I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION

doi:10.1084/jem.132.2.261

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CARRIER FUNCTION IN ANTI-HAPTEN IMMUNE RESPONSES : I. ENHANCEMENT OF PRIMARY AND SECONDARY ANTI-HAPTEN ANTIBODY RESPONSES BY CARRIER PREIMMUNIZATION

David H. Katz M.D.¹, William E. Paul M.D.¹, Edmond A. Goidl ¹, and Baruj Benacerraf M.D.¹

¹ From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014

Preimmunization of either guinea pigs or rabbits to bovinegamma globulin (BGG) prepares the animals for markedly enhancedantibody responses to 2,4-dinitrophenyl-BGG (DNP-BGG). Thisphenomenon is observed both in the primary anti-DNP antibodyresponse to DNP-BGG and in the secondary anti-DNP antibody responseto DNP-BGG in animals primed with DNP-ovalbumin (DNP-OVA). TheBGG preimmunization is most effective if the antigen is administeredas a complete Freund's adjuvant emulsion; in rabbits, a doseof 1 µg of BGG is more effective than a dose of 50 µg,whereas the reverse is true in guinea pigs. Transfusion of homologousanti-BGG sera fails to replace active immunization with BGGin the preparation of animals for these enhanced anti-DNP antibodyresponses. Both the immunoglobulin class and the average associationconstant for $epsi$ -DNP-L-lysine of the anti-DNP antibody producedin these enhanced responses is determined by the mode and timeof immunization with haptenic conjugates and is not appreciablyinfluenced by the nature of the carrier preimmunization.

Thesestudies indicate that the carrier specificity of hapten-specificanamnestic antibody responses is largely due to the interactionof two independent cell associated recognition units, one specializedfor carrier and the other specific for haptenic determinants.

Submitted on March 31, 1970

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