REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS: I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7

doi:10.1084/jem.131.4.629

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REACTIVE LYSIS: THE COMPLEMENT-MEDIATED LYSIS OF UNSENSITIZED CELLS : I. THE CHARACTERIZATION OF THE INDICATOR FACTOR AND ITS IDENTIFICATION AS C7

R. A. Thompson M.B.¹ and P. J. Lachmann Ph.D.¹

¹ From the Department of Experimental Pathology, University of Birmingham, Birmingham, England, and the Department of Pathology, University of Cambridge, Cambridge, England

This paper describes the characteristics of the indicator factor(I) which takes part in reactive hemolysis and its identificationas the seventh component of complement.

I was shown to be abeta globulin with a sediment coefficient of 5.7S and a molecularweight of about 140,000.

Experiments on the depletion of I activitywith anti-I antiserum or with activated R euglobulin showedthat I was a late acting complement component necessary forthe lysis of cells after the EAC142 stage. Complement componentanalysis of purified I fractions excluded all known componentsexcept C7. The physicochemical characteristics of I are compatiblewith published data on C7. The method of quantitation describedrepresents a convenient method of testing for C7.

Submitted on August 25, 1969

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