The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 130, 1145-1160, Copyright © 1969 by The Rockefeller University Press

ARTICLE

THE EIGHTH COMPONENT OF HUMAN COMPLEMENT (C8): ISOLATION, CHARACTERIZATION, AND HEMOLYTIC EFFICIENCY

Jorge A. Manni M.D.1 and Hans J. Müller-Eberhard M.D.1

1 From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma1-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell

. Submitted on July 7, 1969


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