The Journal of Experimental Medicine
StemCell Technologies
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The Journal of Experimental Medicine, Vol 128, 1309-1325, Copyright © 1968 by The Rockefeller University Press

ARTICLE

THE INTERACTION OF HUMAN MONOCYTES AND LYMPHOCYTES

Martin J. Cline M.D.1 and Virginia C. Swett 1

1 From the Cancer Research Institute and the Department of Medicine, University of California San Francisco Medical Center, San Francisco, California 94122

Monocytes isolated from the peripheral blood of tuberculin-positive and tuberculin-negative donors were exposed to PPD, extensively washed, and incubated with autologous or homologous lymphocytes. Lymphocyte transformation was measured morphologically and by incorporation of 14C-labeled thymidine.

Monocytes from tuberculin-positive subjects induced transformation of autologous lymphocytes in 19 of 29 experiments. Studies to define the optimal conditions of exposure to monocytes to PPD and to autologous lymphocytes showed that viable, metabolically intact monocytes are required. A ratio of only 1 monocyte to 100 lymphocytes sufficed to induce transformation; neutrophils were inactive. In general, PPD-sensitized monocytes failed to induce transformation of homologous lymphocytes from either tuberculin-positive or tuberculin-negative subjects. Direct contact between monocytes and lymphocytes was required for consistent transformation, and islands of transforming lymphocytes were observed around a central core of monocytes.

 Submitted on August 7, 1968


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