The Journal of Experimental Medicine
Keystone Symposia
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The Journal of Experimental Medicine, Vol 127, 867-878, Copyright © 1968 by The Rockefeller University Press

ARTICLE

ELECTRON MICROSCOPIC LOCALIZATION OF THE NEPHROTOXIC ANTIBODY IN THE GLOMERULI OF THE RAT AFTER INTRAVENOUS APPLICATION OF PURIFIED NEPHRITOGENIC ANTIBODY-FERRITIN CONJUGATES

Arnold Vogt M.D.1, Hermann Bockhorn 1, Keniti Kozima M.D.1, and Masamichi Sasaki M.D.1

1 From the Hygiene-Institut of the University of Freiburg, Freiburg im Breisgau, West Germany, and the Department of Pathology, University of Kobe, Kobe, Japan

Nephritis in rats was induced by intravenous injection of purified ferritin-conjugated rabbit and duck nephrotoxic globulin.

Using the fluorescent antibody technique, the same capillary pattern was found as that in glomeruli of rats receiving uncoupled nephrotoxic globulin.

Electron microscopy revealed a heavy accumulation of the basement membrane-fixed antibody almost exclusively at the endothelial side. A higher concentration of ferritin was demonstrable in the peripheral basement membrane.

The once-fixed antibody remained at the site of reaction though decreasing with time. The half-disappearance time seemed to be shorter than that of the uncoupled nephrotoxic globulin.

No difference in localization was observed between rabbit and duck antibody.

At least 40 basement membrane-fixed antibody molecules from the rabbit per 3000 mµ2 of filtration surface were needed to cause immediate nephritis. To induce nephritis using duck antibody, a greater number of basement membrane-fixed antibody seemed to be necessary.

No evidence of specific reaction with constituents of glomerular cells was obtained.

 Submitted on November 29, 1967


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