The Journal of Experimental Medicine
StemCell Technologies
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The Journal of Experimental Medicine, Vol 125, 381-392, Copyright © 1967 by The Rockefeller University Press

ARTICLE

TYPE-SPECIFIC POLYSACCHARIDE ANTIGENS OF GROUP B STREPTOCOCCI : II. THE CHEMICAL BASIS FOR SEROLOGICAL SPECIFICITY OF THE TYPE II HCL ANTIGEN



Earl H. Freimer M.D.1

1 From The Rockefeller University, New York

The type-specific antigen of Group B Type II streptococci has been prepared free of other known cell components. This antigen is a capsular polysaccharide containing D-galactose, D-glucose, N-acetyl-D-glucosamine, and a labile component which has not been chemically characterized. Extraction of Type II streptococci with cold TCA yielded this antigen which contained two serological determinants. A partial antigen with only one of the determinants was obtained by extraction with dilute HCl at 100°C.

By means of the quantitative precipitin inhibition technique, a (ß-D-galactopyranoside has been established as the determinant of the HCl-extracted polysaccharide. The second determinant, present solely in the TCA antigen, has not been identified.

 Submitted on October 28, 1966


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