GM AND INV FACTORS IN SUBCLASSES OF HUMAN IGG

doi:10.1084/jem.122.6.1087

3rd Skeletal Biology and Medicine Symposium

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ARTICLE

GM AND INV FACTORS IN SUBCLASSES OF HUMAN IGG

William D. Terry M.D.¹, John L. Fahey M.D.¹, and Arthur G. Steinberg Ph.D.¹

¹ From the Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, and the Department of Biology, Western Reserve University, Cleveland

Human G myeloma (7S $gamma$ ₂-myeloma) proteins were investigated forrelationships between Gm and Inv genetic factors and the differentantigenic types of heavy polypeptide chains ( $gamma$ -chains) and lightpolypeptide chains.

Myeloma proteins were isolated from thesera of 1 Chinese, 60 white and 28 Negro individuals. These89 proteins were tested for eight Gm factors [Gm(a), Gm(x),Gm(b²), Gm(f), Gm(b¹), Gm(b³), Gm(b⁴), and Gm(c)], andtwo Inv factors [Inv(l) and Inv(b)]. Results ofthe tests were correlated with the four $gamma$ -chain subclasses ( $gamma$ _2a, $gamma$ _2b, $gamma$ _2c and $gamma$ _2d) and the two types of light polypeptide chains, $kappa$ -chains (type K or I) and $lambda$ -chains (type L or II) found in humanIgG molecules.

1. Gm factors were limited to myeloma proteinswith heavy polypeptide chains of the $gamma$ _2b- and $gamma$ _2c-subclasses.No Gm factors were detected on $gamma$ _2a- and $gamma$ _2d-myeloma proteins oron a "heavy-chain" disease protein of subclass $gamma$ _2d.

2. $gamma$ _2b-Proteinswere positive for at least one Gm factor and were either Gm(a+),Gm(a + x+), or Gm(b²+ f+).

3. $gamma$ _2c-Myeloma proteins, and one $gamma$ _2c-"heavy-chain"disease protein, were positive for at least one Gm factor andcontained various combinations of factors Gm(b¹), (b²), (b⁴),and (c). Myeloma proteins from 3 Negroes were included in thisgroup.

4. Inv factors (l) and (b) were limited to myeloma proteinswith $kappa$ -light polypeptide chains. These Inv factors were not detectedon proteins with $lambda$ -light polypeptide chains.

5. Most (70 percent) of the $gamma$ _2b- and $gamma$ _2c-proteins with $kappa$ -chains were Inv(l+) orInv(b+). None of the $gamma$ _2a- or $gamma$ _2d-proteins with $kappa$ -chains, however,contained these Inv factors.

Submitted on August 17, 1965

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Steinberg, A. G., Rostenberg, I. (1969). Inv(1) Allotype: Effect of Immunoglobulin G Heavy Chain Subtype on Its Expression. Science 164: 1072-1073 [Abstract]
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