The Journal of Experimental Medicine
StemCell Technologies
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cruchaud, A.
Right arrow Articles by Coons, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cruchaud, A.
Right arrow Articles by Coons, A. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 120, 1061-1074, Copyright © 1964 by The Rockefeller Institute

ARTICLE

STUDIES ON ANTIBODY PRODUCTION : XIII. THE EFFECT OF CHLORAMPHENICOL ON PRIMING IN MICE



Andre Cruchaud M.D.1 and Albert H. Coons M.D.1

1 From the Department of Bacteriology and Immunology, Harvard Medical School, Boston

Young adult mice were primed with 20 Lf (56 µg) of diphtheria toxoid and given a second injection of the same size 40 days later. This procedure produces a reproducible secondary response which can be used as a standard. Chloramphenicol in maximum dosage prevents the unknown process by which the animal is primed for the second response. To be fully inhibitory, the drug must be given from the hour of the first antigen injection in maximum dosage for 2 weeks. A delay of 48 hours in starting the drug allows completion of the priming process, and shorter delays produce partial inhibition. Hence the initiation of priming is a rapid process sensitive to chloramphenicol. Subsequent changes in the cell population necessary for the full development of priming are not sensitive to chloramphenicol.

The secondary antibody response is not inhibited in mice by chloramphenicol at the doses employed.

 Submitted on July 27, 1964


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS