The Journal of Experimental Medicine
Symposium on Dendritic Cells
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hahn, J. J.
Right arrow Articles by Cole, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hahn, J. J.
Right arrow Articles by Cole, R. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 118, 659-666, Copyright © 1963, by The Rockefeller Institute

ARTICLE

STREPTOCOCCAL M ANTIGEN LOCATION AND SYNTHESIS, STUDIED BY IMMUNOFLUORESCENCE

Jerome J. Hahn M.D.1 and Roger M. Cole M.D.1

1 From the United States Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Infectious Diseases, Bethesda

Streptococcal M protein has been studied directly in the intact streptococcal cell by specific immunofluorescence. By this method, it can be seen to be concentrated in or on the cell wall, but cannot be detected in the capsule. The lack of type-specific (but not group-specific) immunofluorescence after trypsinization; and the inhibition of group-specific immunofluorescence by unlabeled type-specific antibody, are observations most compatible with a location of the M antigen determinants on the cell surface superficial to the group antigen.

M antigen is not "resynthesized" after trypsinization of living cells, but appears anew only at sites of new cell wall growth. A limited amount of such growth, leading sometimes to detectable amounts of M in the gross, can take place in deficient media without detectable increases in optical density of the cell population.

 Submitted on June 24, 1963


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS