The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 115, 613-622, Copyright, 1962, by The Rockefeller Institute

ARTICLE

CYTOMETRIC ASSAY OF TOXICITY OF BRUCELLA ANTIGENS FOR SENSITIZED AND NON-SENSITIZED CELLS FROM THE GUINEA PIG

Charles M. Carpenter M.D.1, Morimichi Fukuda M.D.1, and Charles L. Heiskell M.D.1

1 From the Department of Infectious Diseases, School of Medicine, University of California, Los Angeles, and Laboratory Service, Long Beach Veterans Administration Hospital, Long Beach, California

1. A significant cytotoxic effect of trypsinized, sensitized cell cultures was observed when re-exposed to Brucella antigens in vitro.

2. The cytometric assay of the toxic effects of Brucella antigens using trypsinized cells from sensitized animals provides an objective measure of induced cellular hypersensitivity.

 Submitted on October 8, 1961


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