The Journal of Experimental Medicine
Cytokines Montreal 2008
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The Journal of Experimental Medicine, Vol 114, 1063-1078, Copyright, 1961, by The Rockefeller Institute

ARTICLE

THE EFFECTS OF MAMMALIAN AND OTHER CATIONIC POLYPEPTIDES ON THE CYTOCHEMICAL CHARACTER OF BACTERIAL CELLS

John K. Spitznagel M.D.1

1 From the Department of Bacteriology and Immunology, University of North Carolina, School of Medicine, Chapel Hill

Cationic polypeptides interact with bacterial cells of E. coli and B. anthracis. They confer upon the cells some of the characteristics of cationic particles. Since bacterial cells usually behave as anions, acidic dyes at high pH levels differentiate between cells which have and those which have not interacted with cationic polypeptides. Under the conditions of these experiments it appeared that cationic polypeptides tend to be sorbed in highest concentration in the surface layers of the cells. Electrovalent binding to anionic cell components and detergent action are probably among the mechanisms involved in the interaction between the polypeptides such as histones and bacterial cells.

The differential staining of bacterial cells which have interacted with cationic polypeptides is feasible and reasonably selective. It should be useful in determining whether bacterial cells interact with host cationic polypeptides in vitro.

 Submitted on July 31, 1961


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