¹ From the Laboratory of Cell Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Public Health Service, United States Department of Health, Education, and Welfare, Bethesda

A simplified technique has been described for the continuous growth of mammalian cells in suspension culture. The cell population density increased as the rate of input of fresh medium was decreased, and the average generation time was concommittantly prolonged. At relatively high input rates, the population remained stabilized for an indefinite period, but at low flow rates, there was sometimes a cyclical variation in population density. The factor limiting growth rate at input rates of approximately 0.2 volumes per day was not the exhaustion of the medium; but in some experiments a non-dialyzable material appeared which inhibited cell growth.

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This article has been cited by other articles:

• Eagle, H. (1965). Metabolic Controls in Cultured Mammalian Cells: Cultured cells may provide a direct approach to regulation and function in the whole animal. Science 148: 42-51 [Abstract]  

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