¹ From the Division of Dermatology, Department of Internal Medicine, Washington University School of Medicine, and the Barnard Free Skin and Cancer Hospital, St. Louis

Injection of rabbits with Freund's complete adjuvant containing bovine -globulin substituted to near saturation with dinitrophenyl groups leads to the production of large amounts of antibodies specific for the 2,4-dinitrophenyl substituent. These antibodies can be isolated from sera in satisfactory yield (40 per cent) and in a high state of purity (90 per cent specifically precipitable). Hapten dissolution, which was used to screen haptens for their potential usefulness as specific reagents in the purification procedure, provides a means for evaluating, in relative terms, antibody-hapten affinities (in competition with antigen).

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