THE EXPERIMENTAL DISEASE THAT FOLLOWS THE INJECTION OF HUMAN LEPROSY BACILLI INTO FOOT-PADS OF MICE

doi:10.1084/jem.112.3.445

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The Journal of Experimental Medicine, Vol 112, 445-454, Copyright, 1960, by The Rockefeller Institute

ARTICLE

THE EXPERIMENTAL DISEASE THAT FOLLOWS THE INJECTION OF HUMAN LEPROSY BACILLI INTO FOOT-PADS OF MICE

Charles C. Shepard M.D.¹

¹ From the Communicable Disease Center, Public Health Service, United States Department of Health, Education, and Welfare, Montgomery, Alabama

When leprosy bacilli from human patients are inoculated intothe foot-pads of CFW mice, a microscopic granuloma containingacid-fast bacilli develops in a characteristic manner. Thishas been seen in 22 of 22 instances with leprosy bacilli fromnasal washings, in 12 of 16 instances with leprosy bacilli fromskin biopsies, and in none of 16 cases where the nasal washingswere not observed to contain leprosy bacilli.

Quantitative studiesrevealed a relationship between the number of bacilli inoculatedand the time required for the appearance of the lesions. Theincubation period was usually 1 to 2 months when the dose was10^5.5 to 10^6.0 bacilli and about 6 months when the dose wasabout 10³ organisms.

After the development of the lesion, thenumber of bacilli harvested was usually in the range 10^4.5 to10^6.0, regardless of the number inoculated. When the inoculumhas contained 10^2.0 to 10^3.5 acid-fast bacilli, and harvestswere reasonably prompt, there were regular increases of 50-to 1000-fold.

Passage to new groups of mice has been successful11 of 12 times. Most of these were second passages. One strainhas been maintained in 3 passages with a total increase in acid-fastbacilli of 4 x 10⁴-fold. Another strain has been through 4 passageswith a total increase of about 4 x 10⁶-fold.

Cultures on bacteriologicalmedia favorable for the growth of most known mycobacterial specieshave not shown growth of mycobacteria.

Submitted on April 25, 1960

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