The Journal of Experimental Medicine
Keystone Symposia
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The Journal of Experimental Medicine, Vol 111, 831-840, ©Copyright 1960, by The Rockefeller Institute

ARTICLE

STUDIES ON THE PATHOGENESIS OF FEVER : VII. PRELIMINARY CHEMICAL CHARACTERIZATION OF LEUCOCYTIC PYROGEN



Gale W. Rafter Ph.D.1, Robert D. Collins M.D.1, and W. Barry Wood Jr. M.D.1

1 From the Department of Microbiology, The Johns Hopkins University School of Medicine and School of Hygiene and Public Health and the Department of Biochemistry, The Johns Hopkins University School of Hygiene and Public Health, Baltimore

Study of the chemical properties of the pyrogenic component of rabbit polymorphonuclear leucocytes reveals it to contain an essential, non-dialyzable protein which: (a) is precipitated by perchloric acid, (b) is removed by extraction with phenol, (c) is soluble in 50 per cent methanol and 33 per cent saturated ammonium sulfate, and (d) is destroyed by the proteolytic action of both trypsin and pepsin.

By combined chemical and chromatographic techniques the leucocytic pyrogen has been purified approximately 50-fold. The partially purified material contains less than 1 per cent carbohydrate, is resistant to periodate oxidation, is unaffected by extraction with butanol and contains at least two immunologically active components when tested by the Ouchterlony gel-diffusion technique. Its chemical properties distinguish it from other known pyrogenic substances which have been implicated in the pathogenesis of fever.

 Submitted on February 15, 1960


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