The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 111, 429-439, ©Copyright 1960, by The Rockefeller Institute

ARTICLE

IMMUNOLOGIC PRODUCTION OF ANTIANGIOTENSIN : II. PRODUCTION AND DETECTION OF ANTIANGIOTENSIN



Sharad D. Deodhar Ph.D.1

1 From the L. D. Beaumont Memorial Laboratory, The Mount Sinai Hospital, Cleveland

The parenteral administration of benzoylangiotensin II-azo-BGG to rabbits produced an antiserum with antiangiotensin activity.

Antiangiotensin inhibited the biological action of p-aminobenzoylangiotensin II, valine5-angiotensin II (free acid form), isoleucine5-angiotensin II and valine5-angiotensin II (amide form), but it was totally inert towards angiotensin 1.

Antiangiotensin activity was distinguished from that of serum angiotensinase by the following observations: (a) an angiotensinase-free gamma-globulin fraction contained antiangiotensin, (b) angiotensinase inactivated angiotensin II (both the amide and free acid forms) and angiotensin I in contrast to the remarkable specificity for angiotensin II exhibited by antiangiotensin.

Serological demonstration of antiangiotensin included: (a) a comparison of its precipitin reaction with the angiotensin BGG complex with the reaction with BGG alone, (b) the partial inhibition of the precipitin reaction with the angiotensin BGG complex by angiotensin II, (c) a precipitin reaction with a different angiotensin II protein complex (cat serum).

Angiotensin II administered parenterally as the free polypeptide was not antigenic.

 Submitted on November 15, 1959


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