The Journal of Experimental Medicine
BioLegend: Antibody Reagents
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The Journal of Experimental Medicine, Vol 111, 93-106, ©Copyright 1960, by The Rockefeller Institute

ARTICLE

STUDIES ON THE ANTIGENIC PROPERTIES OF COMPLEMENT : I. DEMONSTRATION OF AGGLUTINATING ANTIBODIES AGAINST GUINEA PIG COMPLEMENT FIXED ON SENSITIZED SHEEP ERYTHROCYTES



Paul G. Klein M.D.1 and Peter M. Burkholder M.D.1

1 From the Department of Pathology, The New York Hospital-Cornell Medical Center, New York

Evidence is presented to show that guinea pig complement fixed on sensitized sheep red cells acts as a specific agglutinogen. Agglutinating antibodies that react with cell-fixed complement can be produced by immunizing rabbits with a complex of stromata-amboceptor-complement or with guinea pig serum globulin. These agglutinins can be removed by precipitation with guinea pig serum. They are, therefore, distinct from immunoconglutinins.

Submitted on August 20, 1959


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