The Journal of Experimental Medicine
Torrey Pines Biolabs
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kaplan, M. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kaplan, M. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 107, 341-352, Copyright, 1958, by The Rockefeller Institute for Medical Research New York

ARTICLE

LOCALIZATION OF STREPTOCOCCAL ANTIGENS IN TISSUES : I. HISTOLOGIC DISTRIBUTION AND PERSISTENCE OF M PROTEIN, TYPES 1, 5, 12, AND 19 IN THE TISSUES OF THE MOUSE



Melvin H. Kaplan M.D.1 and With the Technical Assistance of Mary Meyeserian and Audrey Swift

1 From the House of the Good Samaritan, Children's Medical Center, and Department of Bacteriology and Immunology, Harvard Medical School, Boston

A method has been described for the detection of streptococcal antigens in tissues using the indirect immunofluorescent technique. This method has been applied to the histologic distribution in the mouse of M protein of types 1, 5, 12, and 19. Histologic localization of these M proteins was similar, and their rates of disappearance from the tissues were comparable.

The major sites of deposition were the endocardium and adjacent subendocardium of the heart, alveolar walls of the lung, glomerular tufts of the kidney, and reticulo-endothelial cells of liver, spleen, lymph nodes, and adrenal gland. M protein was distributed in considerably lesser concentration in capillary endothelium and connective tissue sites in myocardium, kidney, skin, and gastrointestinal tract. Traces were also present in adrenal cortical cells. It was observed only rarely in cell nuclei.

After injection of 0.5 mg. M protein fraction, the concentration of antigen diminished to undetectable levels in all organ sites by 4 days, except in the renal glomerulus, where traces were visible at 8 days. In mice injected with streptococcal culture intraperitoneally, M protein was detected at sites of focal abscesses in liver and spleen, and on the serous surfaces of these organs.

The histologic distribution of M protein is compared with that described previously for pneumococcal polysaccharide and animal protein. Differences in the extent of distribution and in the characteristics of antigen deposition are pointed out.

 Submitted on September 19, 1957


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS