The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 106, 477-484, Copyright, 1957, by The Rockefeller Institute for Medical Research New York

ARTICLE

MACROGLOBULINEMIA : II. ANTISERA SPECIFIC FOR PATHOLOGICAL MACROGLOBULINS



Leonhard Korngold Ph.D.1 and Gerda Van Leeuwen 1

1 From the Division of Experimental Pathology, Sloan-Kettering Institute for Cancer Research and Sloan-Kettering Division of the Cornell University Medical College, New York

Rabbits were immunized with three highly purified macroglobulins, from patients with macroglobulinemia. The antisera reacted with two macroglobulins with sedimentation constants of 19S and 26S of homologous antigen, and cross-reacted with the 7S and 19S globulins of normal gamma1-globulin and the heterologous pathological 19S macroglobulins. Exhaustive absorption of these antisera with normal gamma-globulins rendered them specific for the homologous macroglobulins. The antigenic properties of the pathological macroglobulins indicate that these proteins are abnormal.

Submitted on May 4, 1957


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