The Journal of Experimental Medicine
R&D Systems
  Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Services
Right arrow Email this article
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new content in the JEM
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fong, J.
Right arrow Articles by Elberg, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fong, J.
Right arrow Articles by Elberg, S. S.
Right arrowPubmed/NCBI databases
Medline Plus Health Information
*Tuberculosis
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
The Journal of Experimental Medicine, Vol 104, 455-465, Copyright, 1956, by The Rockefeller Institute for Medical Research New York

ARTICLE

STUDIES ON TUBERCLE BACILLUS-MONOCYTE RELATIONSHIP : I. QUANTITATIVE ANALYSIS OF EFFECT OF SERUM OF ANIMALS VACCINATED WITH BCG UPON BACTERIUM-MONOCYTE SYSTEM



Jacob Fong Ph.D.1, Patricia Schneider 1, and Sanford S. Elberg Ph.D.1

1 From the Department of Bacteriology, University of California, Berkeley

Studies of the conditions necessary for maintenance of constant cell populations in vitro in the Mackaness type of culture chamber have indicated the importance of preliminary trypsinization of cells and the beneficial effect of 40 per cent rabbit serum in Tyrode's solution. Under these optimal conditions, uninfected suspensions of monocytes exhibited little change in cell numbers over a period of 40 to 72 hours.

Infection of monocytes with the virulent H37Rv strain of tubercle bacillus resulted in an early degeneration of a certain proportion of the cells cultivated in the presence of normal rabbit serum. This degeneration was apparent not only for cells of tuberculin-negative animals but also for those derived from tuberculin-positive animals.

The serum of animals vaccinated with BCG exerted a favorable effect upon the survival of monocytes infected with virulent tubercle bacilli. Treatment with this serum caused a delay in degeneration of infected normal cells (cells of tuberculin-negative rabbits) and a complete inhibition of degeneration of infected immune cells (cells of rabbits vaccinated with BCG).

 Submitted on May 15, 1956


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:



  Home | Help | Feedback | Subscriptions | Archive | Search
TABLE OF CONTENTS