The Journal of Experimental Medicine, Vol 104, 217-231,
Copyright, 1956, by The Rockefeller Institute for Medical Research New York
THE LETHAL EFFECT OF ENDOTOXINS ON THE CHICK EMBRYO
Richard T. Smith M.D.1,
Lewis Thomas M.D.1, and
With the Technical Assistance of Donna G. Gellerman
1 From the Pediatric Heart Research Laboratories, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, and the Department of Pathology, New York University-Bellevue Medical Center, New York
Inoculation of the CAM of the 10-day chick embryo with endotoxin preparations derived from the meningococcus and other Gram-negative microorganisms has been shown to result in multiple hemorrhages and death of the embryo within a few hours. Evidence has been presented to indicate that this lethal effect is specific for the general class of endotoxins derived from Gram-negative bacteria. Susceptibility to endotoxin was maximal in 10-day old embryos, and younger or older embryos showed little or no response. The optimal incubation temperature for the effect of endotoxin was 39.5°C., and embryos incubated at 28°C. were completely protected. The lethal effect was prevented by small amounts of cortisone, hydrocortisone, and 9-alpha fluorohydrocortisone, but not by cholesterol, desoxycorticosterone, or 1-dehydrocortisone.
Submitted on April 3, 1956
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