The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 103, 523-544, Copyright, 1956, by The Rockefeller Institute for Medical Research New York

ARTICLE

STUDIES ON NON-PRECIPITATING ANTIBODY : I. THE CONVERSION OF NON-PRECIPITATING ANTIBODY TO A PRECIPITIN-LIKE MATERIAL IN VITRO



Ludwig A. Sternberger M.D.1, Samuel M. Feinberg M.D.1, and Martha E. Clarke 1

1 From the Allergy Research Laboratory, Department of Medicine, Northwestern University Medical School, Chicago

Brief exposure of serum to alkali in the cold seems to convert non-precipitating antibody to a precipitin-like material. This was disclosed by the following lines of evidence.

1. Immune sera, after addition of antigen and removal of the ensuing precipitates, yielded after alkali treatment more antibody-antigen complex than did sera to which no antigen was added.

2. When immune sera were depleted of precipitating antibody, treated by alkali, and antibody-antigen complex was allowed to precipitate, the resulting supernates formed additional prescipitates upon admixture of specific antigen. No precipitation occurred upon addition of non-specific protein to the treated supemates or the addition of specific antigen to depleted sera not treated by alkali.

3. Alkali-treated immune sera precipitated more antigen than untreated sera.

 Submitted on November 3, 1955


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