THE PREPARATION AND FUNCTION OF THE HYPERTENSIN-CONVERTING ENZYME

doi:10.1084/jem.103.3.295

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The Journal of Experimental Medicine, Vol 103, 295-299, Copyright, 1956, by The Rockefeller Institute for Medical Research New York

ARTICLE

THE PREPARATION AND FUNCTION OF THE HYPERTENSIN-CONVERTING ENZYME

Leonard T. Skeggs Jr. Ph.D.¹, Joseph R. Kahn M.D.¹, and Norman P. Shumway M.D.¹

¹ From the Department of Medicine and Surgery, Veterans Administration Hospital, and the Department of Pathology, Western Reserve University, Cleveland

It has been shown by use of isolated, perfused rat kidneysthat hypertensin II is a potent vasoconstrictor substance whilehypertensin I is not. Hence it would appear that in intact animalsthe pressor activity of hypertensin I results from its rapidconversion to hypertensin II.

An enzyme which effects this conversionhas been procured from horse plasma in a semipurified form bymeans of ammonium sulfate fractionation and isoelectric precipitation.A method is described for estimating the activity of the enzyme.An example of the use of the preparation in converting purifiedhypertensin I to hypertensin II has been described.

Submitted on November 13, 1955

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