The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 103, 285-293, Copyright, 1956, by The Rockefeller Institute for Medical Research New York


ARTICLE

QUANTITATIVE STUDIES ON THE PROPERDIN-COMPLEMENT SYSTEM

Myron A. Leon Ph.D.1 and With the Technical Assistance Of Edna Chapman

1 From the Department of Surgical Research, Saint Luke's Hospital, Cleveland

Quantitative methods have been developed for the estimation of C'3 and properdin. With these methods, the kinetics of the over-all reaction between properdin, zymosan, and C'3 were investigated. The reaction followed first order kinetics, provided zymosan was in excess, and the serum not diluted beyond 1/4. The reaction rate was proportional to the temperature, and an activation energy of 20,500 calories/mole was found.

The distinction between total properdin and available serum properdin is discussed.

Submitted on November 24, 1955


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