The Journal of Experimental Medicine
BioLegend: Antibody Reagents
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The Journal of Experimental Medicine, Vol 102, 631-645, Copyright, 1955, by The Rockefeller Institute for Medical Research New York

ARTICLE

A METHOD FOR COUPLING PROTEIN ANTIGENS TO ERYTHROCYTES : I. DESCRIPTION OF METHOD



Leon R. Cole M.D.1 and Virginia R. Farrell 1

1 From the Departments of Medicine, Peter Bent Brigham Hospital, and Harvard Medical School, Boston

A technique has been described whereby tuberculin PPD is coupled, via tetrazotized benzidne, onto the surfaces of formalinized red blood cells. A stable antigen preparation is thereby obtained, which may be stored at –40°C. The purpose of the present method is to facilitate the study of antibody to tuberculoprotein.

Submitted on August 3, 1955


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