The Journal of Experimental Medicine
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The Journal of Experimental Medicine, Vol 100, 163-180, Copyright, 1954, by The Rockefeller Institute for Medical Research New York

ARTICLE

A STUDY OF THE ANTIGENICITY OF T3 AND T4 COLI-DYSENTERY BACTERIOPHAGES DURING THE VEGETATIVE STAGE OF DEVELOPMENT

Guy T. Barry Ph.D.1

1 From the Laboratories of The Rockefeller Institute for Medical Research

The development of viral neutralizing antibodies in animals injected with T3 or T4 phage is considerably inhibited by the presence of bacterial antigens.

A new procedure has been described to liberate phage from infected E. coli B bacteria by inducing lysis with penicillin.

By immunological means it has been shown that T4-infected cultures of E. coli B, in which phage development has been inhibited with proflavine, contain the viral neutralizing antigen after lysis.

In contrast, it has not been possible to demonstrate by immunological means the appearance of viral neutralizing antigen in E. coli B infected with T3 prior to the appearance of intracellular phage.

 Submitted on April 20, 1954


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